Spawn Preparation (About the Department) (2024)

In the spawn-production process, mycelium from a mushroom culture is placed onto steam-sterilized grain, and in time the mycelium completely grows through the grain. This grain/mycelium mixture is called spawn, and spawn is used to "seed" mushroom compost.

RYE GRAIN SPAWN

250 ml Flasks
50 ml beaker level full of rye grain
1/2 tsp. Calcium Carbonate, powder (lime)
1/4 tsp. Calcium Sulfate (gypsum)
60 ml warm water

Autoclave 35 minutes at 121°C - Fast Exhaust
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1000 ml Flasks
250 ml beaker level full of rye grain
2 tsp. Calcium Carbonate, powder (lime)
1 tsp. Calcium Sulfate (gypsum)
220 ml warm water

Autoclave 45 minutes at 121°C - Fast Exhaust

SHIITAKE RYE SPAWN

Shiitake mushrooms are produced from spawn which is the vegetative growth or pure culture mushroom mycelium on a suitable sterilized substrate such as various agars, grains or wood chips. Formulas listed below were developed for use in producing spawn for synthetic logs. Synthetic logs are usually produced by autoclaving supplemented wood chips in heat-resistant plastic bags containing a breather strip then aseptically inoculating the cooled substrate with spawn. We have successfully produced shiitake mushrooms from synthetic logs inoculated with spawn manufactured as outlined below.

500 ml Flasks
100 ml beaker level full of rye grain
50 ml beaker of hardwood sawdust
1/2 tsp Calcium Sulfate (gypsum)
120 ml of warm tap water
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1000 ml Flasks

250 ml beaker level full of rye grain
100 ml beaker level full of hardwood sawdust
1 tsp Calcium Sulfate (gypsum)
230 ml warm tap water

Use cotton plugs and paper bags over plugs and autoclave for 45 minutes at 121°C - Fast Exhaust

PLEUROTUS RYE SPAWN

In making spawn for Pleurotus, substitute same amount of Calcium Carbonate (lime) INSTEAD of Calcium Sulfate (gypsum).

Spawn Preparation (About the Department) (2024)

FAQs

What is the layout of spawn preparation? ›

The layout plan of a spawn laboratory should have a total built up area of 19x8x3. 6 m (LxBxH). This area will be divided into different work areas like cooking/autoclaving room, inoculation room, and incubation room, washing area, store, office and one cold storage. Cold storage room of 3x3 x3.

What are the raw materials using spawn preparation? ›

For preparation of spawn, the fungus from the culture is then grown on grains or any other suitable medium. spawn. Wheat grains are the most commonly used substrate. The wheat grains are boiled partly or half cooked after soaking overnight so that the grains become slightly soft but do not burst.

What is spawn in mushroom pdf? ›

The seeds used for growing fruiting bodies of mushroom are generally referred to as spawns. More precisely, spawn is the mycelia of mushroom species with its seed substrate. In fact, it is a fungal growth medium impregnated with mycelia fragments of mushroom which acts as inoculum for mushroom cultivation.

What is the process of spawning? ›

The process of spawning typically involves females releasing ova (unfertilized eggs) into the water, often in large quantities, while males simultaneously or sequentially release spermatozoa (milt) to fertilize the eggs.

What is the substrate for spawn preparation? ›

Mother spawn is nothing but the mushroom fungus grown on a grain based medium. Among the several substrate materials tested by TNAU, Coimbatore, sorghum grains are the best substrate for excellent growth of the fungus. Disease- free sorghum grains are used as substrate for growing the spawn materials.

What is spawning stage? ›

Spawning represents the initiation of larval dispersal and places offspring in a specific advective and dispersive flow field, which is critical to determining the outcome of transport and the arrival of offspring at appropriate juvenile habitats. From: Stock Identification Methods (Second Edition), 2014.

How do you prepare soil for casing? ›

The soil used for casing process should be free of stones and stubble and has to be sterilized before casing. The soil is taken in a mud pot or a vessel and steamed in an autoclave or pressure for 45 minutes. (Soil can also be mixed with a little quantity of water and sterilized for this purpose).

What is spawn method? ›

Spawn in computing refers to a function that loads and executes a new child process. The current process may wait for the child to terminate or may continue to execute concurrent computing. Creating a new subprocess requires enough memory in which both the child process and the current program can execute.

How is bed spawn prepared? ›

Bed spawns:

The method of preparation of bed spawn was same as that of mother spawn. The cooking, filling and sterilization were similar to that of mother spawn. Instead of saline bottles, polypropylene bags were used. After sterilization, the bags are taken for inoculation.

What is the process of making spawn? ›

In the spawn-production process, mycelium from a mycelial culture is placed onto steam-sterilized grain, and in time the mycelium completely grows through the grain. This grain/mycelium mixture is called spawn, and spawn is used to "seed" mushroom compost.

What are the equipment required for spawn production? ›

A grain boiler is operated by 6 kW electric power. The mixer is for mixing boiled grains with chalk powder. The bag filler for spawn and innoculator for mother spawn has also been developed. These machineries have been developed for 100 kg per day of spawn production.

How to prepare spawn on large scale? ›

The steps are as follows:
  1. Selection of the healthy Pleurotus ostreatus species.
  2. Preparation of potato dextrose agar/malt extract agar media.
  3. Pure culture of selected mushroom should be obtained.
  4. Preparation of substrate for spawn culture.
  5. Mother spawn preparation.
  6. Multiplication of spawn at commercial level.
Jan 1, 2023

How do you make a mushroom spawn step by step? ›

How to Grow Oyster Mushroom Spawn (Low Tech)
  1. Step 1: Materials. ...
  2. Step 2: Prepare Clean Room. ...
  3. Step 3: Prepare Jars. ...
  4. Step 4: Prepare Grain. ...
  5. Step 5: Sterilisation. ...
  6. Step 6: Inoculation I (Grain Spawn Transfer) ...
  7. Step 7: Inoculation II (Agar Tissue Culture Transfer) ...
  8. Step 8: Inoculation III (Liquid Inoculation Methods)

What are the steps in the mushroom process? ›

The six steps are Phase I composting, Phase II composting, spawning, casing, pinning, and cropping. These steps are described in their naturally occurring sequence, emphasizing the salient features within each step. Compost provides nutrients needed for mushrooms to grow.

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